FIG. 3.

Northern blotting indicates a significant decrease in EhSTIRP gene expression when exposed to dsRNA targeting the 3′ end of the EhSTIRP gene family. (A) Northern blot analysis shows substantial down-regulation of EhSTIRP expression in the presence of dsRNA targeted to the 3′ end of the EhSTIRP gene family (parasites selected at 48 μg/ml G418). A PCR product from the 3′ end of the gene family that should detect EhSTIRP1-3 was used as a probe. RNA probed with an actin probe and methylene blue-stained rRNA (grayscale) are shown as loading controls. (B) Gene-specific oligonucleotide probes were also used and indicated that the expression level of each full-length EhSTIRP was decreased in the dsRNA-expressing parasites. RNA probed with actin and methylene blue-stained rRNA (grayscale) are shown as loading controls.