TABLE 2.
Filamentous growth in liquid mediaa
| Temp (°C) | Medium | Growthb
|
|||
|---|---|---|---|---|---|
| Wild type | sfl1/sfl1 mutant | sfl1/sfl1 flo8/flo8 mutant | sfl1/sfl1+FLO8 mutant | ||
| 37 | Lee's, pH 4 | − | +++ | − | ++++ |
| Lee's, pH 8 | +++ | ++++ | − | ++++ | |
| YPD | + | ++++ | − | ++++ | |
| YPD + serum | +++ | ++++ | − | ++++ | |
| SCLD | +++ | ++++ | − | ++++ | |
| SLAD | +++ | ++++ | − | ++++ | |
| 30 | YPD | − | − | − | ++ (30) |
| YP | − | ++ (20) | − | ++ (50) | |
| Spider | − | ++++ (100) | − | ++++ (100) | |
| SCD | − | +++ (30) | − | +++ (80) | |
| SCLD | − | +++ (70) | − | +++ (90) | |
| SLAD | − | +++ (60) | − | +++ (90) | |
| Lee's, pH 4 | − | ++ (10) | − | ++ (30) | |
| Lee's, pH 8 | − | +++ (60) | − | +++ (80) | |
| YPD + serum | − | ++++ (100) | − | ++++ (100) | |
| SCD + serum | − | ++++ (100) | − | ++++ (100) | |
| Lee's + serum | − | ++++ (100) | − | ++++ (100) | |
| 25 | YPD | − | − | − | − |
| YP | − | − | − | − | |
| SCD | − | − | − | − | |
| SCLD | − | + (2) | − | + (10) | |
| SLAD | − | + (5) | − | + (30) | |
| Spider | − | + (5) | − | + (20) | |
| Lee's | − | − | − | − | |
| YPD + serum | − | − | − | − | |
| YP + serum | − | − | − | − | |
| SCD + serum | − | − | − | − | |
| SCLD + serum | − | ++ (50) | − | +++ (80) | |
| SLAD + serum | − | ++ (30) | − | +++ (80) | |
| Spider + serum | − | ++ (30) | − | +++ (70) | |
| Lee's + serum | − | − | − | − | |
a
Wild-type (CAI4+pBES116), sfl1/sfl1 (CAL2+pBES116), sfl1/sfl1 flo8/flo8 (CAL4+pBES116), and sfl1/sfl1+FLO8 (CAL2+pBA1-FLO8) strains were grown in liquid media. The media used for yeast and hyphal growth were as described in Materials and Methods.
b
−, yeast growth; +, filamentous growth. The level of cell filamentous growth is indicated by the number of + symbols. The percentage of hyphal cells in a given cell population counted is in parentheses.