FIG. 4.
Effects of TbUBP1/2 up- and downregulation on cell growth. Cells were seeded at between 0.1 and 5 × 105 cells/ml and diluted as required. Cultures were bloodstream-form (A and C) or procyclic-form (B and D) trypanosomes and were grown either with (open symbols and dotted lines) or without (closed symbols) 100 ng of tetracycline/ml as indicated within each panel. Upward arrowheads indicate overexpression, and downward arrowheads indicate depletion. Expression of TcUBP1 also inhibited growth (not shown).