Skip to main content
. 2007 Aug 31;6(11):2147–2156. doi: 10.1128/EC.00262-07

FIG. 3.

FIG. 3.

Immuno-electron microscopy localization of TgSERCA versus GFP-HDEL in T. gondii. TgSERCA localized to perinuclear ER in both intracellular and extracellular parasites. In extracellular parasites, SERCA labeling extended into the apical region of parasites where it was associated with vesicular structures (apical region indicated by arrows). (A) Intracellular T. gondii immunolabeled with mouse anti-TgSERCA antibody. (B) Extracellular T. gondii immunolabeled with mouse anti-TgSERCA antibody. (C) Intracellular T. gondii expressing GFP-HDEL immunolabeled with rabbit anti-GFP antibody. (D) Extracellular T. gondii expressing GFP-HDEL immunolabeled with rabbit anti-GFP antibody. (E) Intracellular T. gondii immunolabeled with isotype control antibody. N, parasite nucleus; DG, dense granules; G, position of the Golgi. Scale bars, 0.5 μm. Note that the variable size of gold particles seen here is due to the silver enhancement technique. To facilitate identification of gold particles, representative clusters are highlighted by red circles.