FIG. 2.
YcfN allows a ΔcobU mutant to salvage Cbi. Results are shown for Cbl-dependent growth of serovar Typhimurium strains in NCE glycerol medium containing (CN)2Cbi (15 nM) at 37°C. Strains used are methionine auxotrophs that must synthesize Cbl to satisfy their requirement for methionine. Growth is reported as OD595 as a function of time. Strains are indicated by their relative genotypes. Strains used were TR6583 (cobU+ ycfN+), JE8268 (ΔcobU ΔycfN), JE8309 (ΔcobU/pcobY+), JE8310 (ΔcobU ΔycfN/pcobU+), JE8314 [(ΔcobU ΔycfN/pcobY+ pET16b; empty-vector control)], and JE8315 (ΔcobU ΔycfN/pcobY+ pycfN+).