TABLE 1.
Strains and plasmids used in these studies
| Strain or plasmid | Relevant genotype and/or description | Reference or source |
|---|---|---|
| Strains | ||
| Salmonella entericaa | ||
| TR6583 (formerly SA2929) | metE205 ara-9 | K. Sanderson via J. Roth |
| Derivatives of TR6583 | ||
| JE7127 | ΔcobUST ycfN121::Tn10d(tet+)/pCOBY35 | |
| JE7178 | ΔcobUST272/pCOBY35 | Laboratory collection |
| JE8249 | ΔcobU1315 | |
| JE8268 | ΔcobU1315 ΔycfN112 | |
| JE8308 | ΔcobU1315/pSU39 | |
| JE8309 | ΔcobU1315/pCOBY38 | |
| JE8310 | ΔcobU1315 ΔycfN112/pJO52 | |
| JE8312 | ΔcobU1315 ΔycfN112/pCOBY38 | |
| JE8313 | ΔcobU1315 ΔycfN112/pCLK740 | |
| JE8314 | ΔcobU1315 ΔycfN112/pCOBY38 pET-16b | |
| JE8315 | ΔcobU1315 ΔycfN112/pCOBY38 pCLK740 | |
| JE8407 | ΔcobU1315 ΔycfN112/pCLK740 pTara | |
| JE8408 | ΔcobU1315 ΔycfN112/pET-16b pTara | |
| Escherichia coli | ||
| BL21(DE3) | B F−ompT hsdS (rB− mB−) dcm gal λ(DE3) | Invitrogen |
| Plasmids | ||
| pBAD33 | Cloning vector; cat+ | 14 |
| pCLK740 | E. coli ycfN+ cloned into plasmid pET-16b | 18 |
| pCOBA17 | cobA+ in pET-15b; encodes His6-CobA | 9 |
| pCOBY35 | S. enterica cobST+Methanosarcina mazei cobY+ (cobYST+) cloned into plasmid pBAD33 | Laboratory collection |
| pCOBY38 | M. mazei cobY+ cloned into plasmid pSU39 | 31 |
| pET-16b | N-terminal His10 tag vector; bla+ | Novagen |
| pGP1-2 | T7 RNA polymerase+kan+ | 24 |
| pJO52 | cobU+ cloned into plasmid pT7-7 | 19 |
| pSU39 | Cloning vector; kan+ | 2 |
| pT7-7 | Cloning vector; bla+ | 24 |
| Ptara | T7 RNA polymerase+cat+ | 34 |
a
All strains are derivatives of S. enterica serovar Typhimurium strain LT2. Unless otherwise stated, strains were constructed for this study.