TABLE 1.
Plasmids and strains
| Plasmid or strain | Description of construction | Reference or source |
|---|---|---|
| Plasmids | ||
| pLL28 | Temperature-sensitive vector | 20 |
| pLL33 | Translational blaZ fusion vector | 19 |
| pLL35 | Transcriptional blaZ fusion vector | 19 |
| pLL38 | Transcriptional xylE fusion vector | This study |
| pZC3618 | Pcap5::xylE transcriptional fusion in pLL38 | This study |
| pZC3419 | Psbc::xylE transcriptional fusion in pLL38 | This study |
| pZC3624 | pZC3618 carrying sbcDC operon | This study |
| pCL8074 | Pcap5/8::xylE transcription fusion in pLC4 | 25 |
| pAM3175 | Pcap5/8::blaZ translational fusion in pLL33 | 19 |
| pAM3176 | Pcap5/8::blaZ transcriptional fusion in pLL35 | 19 |
| pTL3562 | Parl-blaZ transcriptional fusion in pLL35 | This study |
| pTL3564 | Pagr-3::blaZ transcriptional fusion in pLL35 | This study |
| pTL3580 | PmgrA-blaZ transcriptional fusion in pLL35 | This study |
| pTL3573 | pCL15 containing arlR | 19 |
| Strains | ||
| RN4220 | 8325r− | 14 |
| Newman | CP5 strain | T. Foster |
| CYL6619 | Newman ΔsbcD::cat | This study |
| CYL6620 | Newman ΔsbcC::cat | This study |
| CYL6621 | Newman ΔsbcDC::cat | This study |
| CYL1164 | Newman ΔarlR | 19 |
| CYL6851 | Newman ΔsbcDC::catΔarlR | This study |