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. 2007 Aug 10;189(20):7426–7435. doi: 10.1128/JB.00464-07

FIG. 1.

FIG. 1.

Purification of EHEC H7 flagella. A transmission electron micrograph of EPEC E2348/69 (O127:H6) (A) and EHEC EDL933 (O157:H7) (B) grown in 1% tryptone expressing flagella (arrows) is shown. (C) Transmission electron micrograph of purified flagella from EDL933. (D) SDS-PAGE analysis of purified H7 and H6 in 16% SDS-PAGE gel. Both flagella are composed of an approximately 60-kDa subunit. Immunogold labeling of EPEC H6 (E) and EHEC H7 (F) flagella is shown. The inset is a high magnification of the decorated flagella. (G) Western blot analysis of whole bacterial cells by use of antibodies against H6 or H7 flagella demonstrating the specificity of antibodies utilized. Molecular mass markers (in kDa) are indicated on the left.