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Demonstration of binding of H7 flagella to mucins and bovine mucus. Purified H7 flagella were incubated with mucins I (A) and II (B) and bovine mucus (C) immobilized onto glass coverslips. Flagella were stained by immunofluorescence using primary antibodies against flagella from chicken and secondary goat anti-chicken antibodies conjugated to Alexa Fluor 594 (red). The control slide was precoated with albumin only (D).
