TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s)a | Source or reference |
|---|---|---|
| Strains | ||
| S. thermophilus | ||
| LMD-9 | Wild type | ATCCb |
| LF101 | LMD-9 ΔblpRSt | This study |
| LF102 | LMD-9 ΔblpHSt | This study |
| LF103 | LMD-9 Δ(blpRSt-blpHSt) | This study |
| LF104 | LMD-9 Δ(blpDSt-blpXSt) | This study |
| LF105 | LMD-9 Δ(blpDSt-blpFSt) | This study |
| LF106 | LMD-9 Δ(blpGSt-blpXSt) | This study |
| LF107 | LMD-9 ΔblpBSt | This study |
| LF108 | LMD-9 Δ(blpASt-blpBSt) | This study |
| LMG18311 | Wild type | 8 |
| CNRZ1066 | Wild type | 8 |
| E. coli | ||
| TG1 | supE hsdΔ5 thi Δ(lac-proAB) F′[traD36 proAB+ lacIqlacZΔM15] | 42 |
| EC1000 | Kmr RepA+; MC1000 containing a copy of the repA gene of pWV01 in its chromosome | 33 |
| Plasmids | ||
| pMG36e | Emr; E. coli-S. thermophilus shuttle vector; contains the P32 promoter | 44 |
| pGIBG001 | Emr; pMG36e where a NcoI restriction site has been introduced in order to allow the cloning of genes in translational fusion with the P32 promoter | This study |
| pGILF001 | Emr; pGIBG001 with a 0.16-kb insert containing the StblpC ORF of S. thermophilus LMG18311 in translational fusion the P32 promoter | This study |
| pGILF002 | Emr; pGIBG001 with a 0.16-kb insert containing the StblpC ORF of S. thermophilus LMD-9 in translational fusion the P32 promoter | This study |
| pGhost9 | Emr Ts | 35 |
a
Emr and Kmr indicate resistance to erythromycin and kanamycin, respectively, and Ts indicates a temperature-sensitive replicative plasmid.
b
American Type Culture Collection, Rockville, MD.