FIG. 1.
Expression of spoIIIA-lacZ fusions. (A) β-Galactosidase accumulation in cultures of strains containing various regions of the spoIIIA locus, including spoIIIAH (⧫), spoIIIAGH (•), spoIIIAFGH (▴), and spoIIIA′EFGH (▪), transcriptionally fused to lacZ. (B) Comparison of P1spoIIIA and P2spoIIIA activities. β-Galactosidase activity was measured in strains containing P1spoIIIA (○) and spoIIIAFGH, which contains P2spoIIIA (▴), transcriptionally fused to lacZ. The data are the averages of three replicates. The x axis indicates the time (in hours) before and after the onset of sporulation (zero time).