FIG. 4.
Primer extension of analysis P2spoIIIA. Total RNA was isolated 4 h after the onset of sporulation from three B. subtilis strains (ΔspoIIID [lane a], ΔsigE [lane b], and wild type [lane c]) harboring a P2spoIIIA-lacZ fusion at amyE. The primer extension product and putative transcription start point are indicated by an arrow on the left and by +1 on the right. The −35 and −10 regions are also indicated to the right of the DNA sequence of the expanded region. Lanes d, e, f, and g contained the dideoxy sequencing reactions for G, A, T, and C, respectively; the same primer was used for sequencing and for primer extension.