TABLE 2.
B. subtilis strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| JH642 | trpC2 pheA1 | J. Hoch |
| AOB114 | MB24 gerE::kan | Lab stock |
| RL2045 | ΔspoIIIAG | P. Stragier |
| RL2046 | ΔspoIIIAH | P. Stragier |
| CGB125 | amyE::spoIIIAH | This study |
| CGB126 | amyE::spoIIIAGH | This study |
| CGB127 | amyE::spoIIIAFGH | This study |
| CGB128 | amyE::spoIII′AEFGH | This study |
| CGB129 | RL2046 amyE::spoIIIAH | This study |
| CGB130 | RL2046 amyE::spoIIIAGH | This study |
| CGB131 | RL2046 amyE::spoIIIAFGH | This study |
| CGB132 | RL2046 amyE::spoIII′AEFGH | This study |
| CGB133 | RL2045 amyE::spoIIIAH | This study |
| CGB134 | RL2045 amyE::spoIIIAGH | This study |
| CGB135 | RL2045 amyE::spoIIIAFGH | This study |
| CGB136 | RL2045 amyE::spoIIIA′EFGH | This study |
| CGB142 | amyE::spoIIIAH-lacZ | This study |
| CGB143 | amyE::spoIIIAGH-lacZ | This study |
| CGB144 | amyE::spoIIIAFGH-lacZ | This study |
| CGB145 | amyE::spoIIIA′EFGH-lacZ (last 200 bp of spoIIIAE) | This study |
| CGB147 | amyE::P1spoIIIA-lacZ | This study |
| CGB158 | amyE::spoIIIAFGH-lacZ (42 bp of spoIIIAH) | This study |
| CGB159 | amyE::spoIIIAFG-lacZ (42 bp of spoIIIAG) | This study |
| CGB160 | amyE::spoIIIAF-lacZ | This study |
| CGB161 | amyE::spoIIIAF-lacZ (200 bp at 3′ end of spoIIIAF deleted) | This study |
| CGB162 | amyE::spoIIIAF-lacZ (400 bp at 3′ end of spoIIIAF deleted) | This study |
| CGB163 | amyE::spoIIIA′EFGH-lacZ (42 bp of spoIIIAH) | This study |
| CGB164 | amyE::spoIIIA′EFG-lacZ (42 bp of spoIIIAG) | This study |
| CGB165 | amyE::spoIIIA′EF-lacZ (200 bp at 3′ end of spoIIIAF deleted)a | This study |
| CGB166 | amyE::spoIIIA′EF-lacZ (400 bp at 3′ end of spoIIIAF deleted) | This study |
| CGB167 | amyE::spoIIIA′EF-lacZ (600 bp at 3′ end of spoIIIAF deleted) | This study |
| CGB168 | amyE::spoIIIA′E-lacZ (last 200 bp of spoIIIAE) | This study |
| CGB172 | amyE::spoIIIAF-lacZ (100 bp at 5′ end of spoIIIAF deleted) | This study |
| CGB173 | amyE::spoIIIAF-lacZ (200 bp at 5′ end of spoIIIAF deleted)b | This study |
| CGB174 | amyE::spoIIIAF-lacZ (300 bp at 5′ end of spoIIIAF deleted) | This study |
| CGB175 | spoIIIAF Campbell | This study |
| CGB176 | spoIIIAE Campbell | This study |
| CGB177 | spoIIIAD Campbell | This study |
| CGB178 | spoIIIAC Campbell | This study |
| CGB179 | spoIIIAB Campbell | This study |
| CGB187 | gerR::spec amyE::P1spoIIIA | This study |
| CGB188 | spoIIID::kan amyE::P1spoIIIA | This study |
| CGB189 | sigE::spec amyE::P1spoIIIA | This study |
| CGB190 | sigK::tet amyE::P1spoIIIA | This study |
| CGB191 | gerE::kan amyE::P1spoIIIA | This study |
| CGB192 | gerR::spec amyE::P2spoIIIA | This study |
| CGB193 | spoIIID::kan amyE::P2spoIIIA | This study |
| CGB194 | sigE::spec amyE::P2spoIIIA | This study |
| CGB195 | sigK::tet amyE::P2spoIIIA | This study |
| CGB196 | gerE::kan amyE::P2spoIIIA | This study |
| CGB197 | amyE::PsspE-lacZ | This study |
| CGB198 | CGB175 amyE::PsspE-lacZ | This study |
| CGB202 | amyE::P2spoIIIA-lacZ (−30 C-to-T mutation) | This study |
| CGB203 | amyE::P2spoIIIA-lacZ (−12/−11 CA-to-GT mutation) | This study |
| CGB206 | amyE::spoIIIAFGH-lacZ (−12/−11 CA-to-GT mutation) | This study |
| CGB207 | amyE::spoIIIAFGH (−12/−11 CA-to-GT mutation) | This study |
| CGB208 | RL2045 amyE::spoIIIAFGH (−12/−11 CA-to-GT mutation) | This study |
| CGB209 | RL2046 amyE::spoIIIAFGH (−12/−11 CA-to-GT mutation) | This study |
| CGB225 | spoIIIAFGH (−12/−11 CA-to-GT mutation) | This study |
| CGB226 | CGB197 spoIIIAFGH (−12/−11 CA-to-GT mutation) | This study |
| CGB227 | CGB225 amyE::spoIIIAFGH-lacZ | This study |
a
This site was used as the 3′ end for all remaining experiments.
b
The fragment of DNA from base 200 to base 415 of spoIIIAF is referred to as P2spoIIIA.