TABLE 3.
Oligonucleotide primers used for PCR, sequencing, and mutagenesisa
| Primer | Sequence (5′ to 3′) |
|---|---|
| spoIIIAH-rev-EcoRI | CACACACAGAATTCTTTAGCGGGCTTTTTTCCCTCATTCTTA |
| spoIIIAH-for-BamHI | CACACACAGGATCCCTTAAAAAACAAACCGTTTGGCTATT |
| spoIIIAG-for-BamHI | CACACACAGGATCCAATAAAAACGGATTATGGAATGTA |
| spoIIIAF-for-BamHI | CACACACAGGATCCAGTTTTTTAACGGAATGGCTTACC |
| spoIIIAE-for-BamHI | CACACACAGGATCCGTAATCAGCGCCTCTTTACT |
| spoIIIAH-rev-BamHI | CACACACAGGATCCTTTAGCGGGCTTTTTTCCCTCATTC |
| spoIIIAH-for-EcoRI | CACACACAGAATTCCTTAAAAAACAAACCGTTTGGCTATT |
| spoIIIAG-for-EcoRI | CACACACAGAATTCAATAAAAACGGATTATGGAATGTA |
| spoIIIAF-for-EcoRI | CACACACAGAATTCAGTTTTTTAACGGAATGGCTTACC |
| spoIIIAE-for-EcoRI | CACACACAGAATTCGTAATCAGCGCCTCTTTACT |
| spoIIIAH-rev-fuse-BamHI | CACACACAGGATCCTAAGACTGAGCATTGTTAATAGCCAAA |
| spoIIIAG-rev-fuse-BamHI | CACACACAGGATCCTAAGAGACTGCTTTTTCAATACATTCCA |
| spoIIIAF-rev-BamHIA | CACACACAGGATCCTTTCATTGCCGACACTCTC |
| spoIIIAF-rev-BamHIB | CACACACAGGATCCTGGCCATATACACGCTGATTGTTTT |
| spoIIIAF-rev-BamHIC | CACACACACAGGATCCTCTCTGACTGCCCGTTTTT |
| spoIIIAE-rev-BamHI | CACACACAGGATCCATGAGAGACACAATGGCGAGA |
| spoIIIAF-for-EcoRIA | CACACACAGAATTCATGGTAGTCAGCCTGCTCTTGATT |
| spoIIIAF-for-EcoRIB | CACACACAGAATTCAAACGGGCAGTCAGAGTCT |
| spoIIIAF-for-EcoRIC | CACACACAGAATTCAATGGCTGTCCAACTAAA |
| lacZ-rev2 | ATCTTACGTCAGTAACTTCCACAGT |
| PspoIIIA-for-EcoRI | CACACACAGAATTCACGGCAGCAATTGTCATGCTTGTGA |
| PspoIIIA-rev-BamHI | CACACACAGGATCCATTGGCTTCTTTAAAATGTATGATGTGAG |
| spoIIIAF-for-EcoRV | CACACACAGATATCTCACGATGATGATGAAATGAAGGA |
| spoIIIAF-rev-BamHI | CACACACAGGATCCGAAGAGACTGCTTTTTCAATACAT |
| spoIIIAE-for-EcoRV | CACACACAGATATCTGTCATAACCGAAAGGAGGCGGTA |
| spoIIIAE-rev-BamHI | CACACACAGGATCCACAACACGAATGGTGGTAAGC |
| spoIIIAD-for-EcoRV | CACACACAGATATCAGCTGTGTTTTTATTCCAAGGATAGG |
| spoIIIAD-rev-BamHI | CACACACAGGATCCTTCAATCTACCGCCTCCTTT |
| spoIIIAC-for-EcoRV | CACACACAGATATCGATGTAAACGTGAGGGGAGCAA |
| spoIIIAC-rev-BamHI | CACACACAGGATCCAATGTCAATCTGCAAGCCCCCCTAT |
| spoIIIAB-for-EcoRV | CACACACAGATATCAAATTTATGACAAAGACGGAAATGTG |
| spoIIIAB-rev-BamHI | CACACACAGGATCCTTTATTTTTGCTCCCCTCACGTTA |
| gerR-for-del-EcoRV | CACACACAGATATCCTGAACAAGAAAAAGGAGCTGCTC |
| gerR-rev-del-EcoRI | CACACACAGAATTCAGTCCAAGCATCTTGTCTTGTAATGGT |
| gerR-for-del-BamHI | CACACACAGGATCCATGAAAAAAGCGGCTCAAGAA |
| gerR-rev-del-SalI | CACACACAGTCGACTCAAAGAATACGGCATTCAGGA |
| spoIIID-for-del-EcoRI | CACACACAGAATTCGAGTCATTGGTCCGATCGTA |
| spoIIID-rev-del-BamHI | CACACACAGGATCCTGTTCGCTCTTTGATGTAATCGTGCA |
| spoIIID-for-del-PstI | CACACACACTGCAGGAAGGAGAGCCTGTTCAGCAAT |
| spoIIID-rev-del-SphI | CACACACAGCATGCTTTGAGAACAGGCCTTTTACA |
| sigE-for-del-EcoRV | CACACACAGATATCGTCAGCGTGCAGGCAGATT |
| sigE-rev-del-EcoRI | CACACACAGAATTCCAGCAGCTTATACCAGAGGTGCGTCAA |
| sigE-for-del-BamHI | CACACACAGGATCCAAAGAGTTCAACAAAATGGTG |
| sigE-rev-del-SalI | CACACACAGTCGACTTGTAAGCGATGTCCC |
| sigK-for-del-EagI | CACACACACGGCCGACAATAAACAGCACTCTGGTACC |
| sigK-rev-del-BamHI | CACACACAGGATCCAACAAAGCCGAGCGCTGCGAAAA |
| sigK-for-del-EcoRI | CACACACAGAATTCATCTTCAAGAGTTAAGTTATCGCACCGAT |
| sigK-rev-del-HindIII | CACACACAAAGCTTGGTTTGGTTAGATGCGGAAAATGC |
| AF35for | CAAAAAAAATAGAAACACAAGCTTCCCAGCG |
| AF35rev | CGCTGGGAAGCTTGTGTTTCTATTTTTTTTG |
| AF10for | CAAGCTTCCCAGCGCGGTTATATTCTAGAAGAAATGG |
| AF10rev | CCATTTCTTCTAGAATATAACCGCGCTGGGAAGCTTG |
| sspE-for-HindIII | CACACACAAAGCTTACGCATGGTCGAAATTAAAGAC |
| sspE-rev-BamHI | CACACACAGGATCCTGTTATCACCTCCACGGTCA |
a
Restriction enzyme sites are indicated by bold type, and base changes in QuikChange primers are indicated by underlining.