FIG. 2.

Complementation of an ftsQ1(Ts) strain by plasmid-encoded FtsQ. A: Complementation experiment in liquid culture. MJC129 [ftsQ1(Ts)] with different plasmids was grown overnight at the permissive temperature (30°C), diluted to an OD₆₆₀ of 0.01 in prewarmed LB medium supplemented with 0.02% arabinose, and grown at the nonpermissive temperature (42°C). Plasmids were as follows: pBAD24 (⧫), pBAD24-FtsQ (▪), pLD104 (FFQ) (⋄), pBAD24-FtsQL29R (▵), pBAD24-FtsQL32R (▴), pBAD-FtsQL29/32R (○), pBAD24-FtsQL29/32R;V38P (•), and pBAD24-FtsQV38P (□). B: Complementation experiment on plates. Exponentially growing cells of MJC129 [ftsQ1(Ts)] with different plasmids, grown at 30°C, were spotted on plates containing 0.01% arabinose and incubated overnight at the permissive (30°C) or nonpermissive (42°C) temperature. wt, wild type.