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Localization to the cell division site is abolished in the L29/32R double mutant. Phase-contrast and fluorescence images are of cells expressing GFP fusions to FtsQ (A), FtsQL29R (B), FtsQL32R (C), FtsQV38P (D), FtsQL29/32R (E), and FtsQL29/32R;V38P (F), grown to steady state at 28°C in GB1 medium. Some division sites are indicated with arrowheads. Bar (same for all), 2 μm.
