FIG. 3.
Autokinase and phosphatase activities of KdpD derivatives with single amino acid replacements. Kinase (gray bars) and phosphatase (black bars) activities were determined as described in Materials and Methods. Data are presented as percentages of the initial rates relative to wild-type KdpD. For wild-type KdpD (100% value) the autokinase activity was 0.1 pmol of KdpD∼P formed min−1, and the phosphatase activity was 0.02 pmol of Pi released from KdpE∼P min−1. The data presented represent average values obtained in at least three independent experiments.