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Anti-NflD and anti-NflH Western blots of the elution buffer fraction of the cross-linked anti-NflD IgG-protein-A agarose column. Lane 1, molecular mass markers (250, 150, 100, 75, 50, 37, 25, and 20 kDa); lane 2, anti-NflD (1:20,000 dilution) primary/Novagen goat anti-rabbit IgG-AP conjugate; lane 3, anti-NflH (1:1,000 dilution) primary/Novagen goat anti-rabbit IgG-AP conjugate.
