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. 2007 Jul 27;189(20):7392–7398. doi: 10.1128/JB.00876-07

FIG. 2.

FIG. 2.

Coomassie-stained 10% acrylamide SDS-PAGE of M. jannaschii proteins remaining tightly associated to a cross-linked polyclonal anti-NflD IgG-protein-A agarose after extensive washing with elution buffer and 2 M NaCl. MJ1157, a 100.3-kDa protein (NCBI accession no. E64444); MJ0861, a 50.03-kDa protein (NCBI accession no. E64407); MJ1210, a 28.68-kDa protein (NCBI accession no. A64451). Lane 1, molecular mass markers (250, 150, 100, 75, 50, 37, 25, and 20 kDa); lane 2, proteins extracted from the slurry of column matrix using trichloracetic acid-acetonitrile-SDS-sample buffer as described in the text.