TABLE 1.
Bacterial strains, plasmids, bacteriophages, and bacteriocins used in this work
| Bacterial strain, plasmid, or bacteriophage or bacteriocin | Comments | Reference(s) or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| SY327λpir | λ(lac pro) argE(Am) rif nalA recA56 (λpir) | 50 |
| Sm10λpir | thi thr leuB tonA lacY supE recA::RP4-2-Tc::Mu-Km (λpir) | 61 |
| S17-1 | thi pro hsdR hsdM+recA::RP4-2-Tc::Mu-Km::Tn7 Strr | 61 |
| S17-1λpir | thi pro hsdR hsdM+recA::RP4-2-Tc::Mu-Km::Tn7 Strr (λpir) | 29 |
| HB101/pRK2013 | Triparental conjugation helper strain; Kmr | 31 |
| C600 | thi thr leuB tonA lacY supE | 6 |
| Y. enterocolitica | ||
| Ruokola/71 | Patient isolate | 62 |
| Ruokola/71-c | Spontaneous virulence plasmid-cured derivative of Ruokola/71 | 62 |
| YeO9-OC-R | Phage φR1-37-resistant spontaneous OC-negative derivative of YeO9-R1 | This work |
| YeO9-R1 | Δper::KmGB; rough (OPS negative); Kmr derivative of Ruokola/71 | This work |
| YeO9-c-R3 | Δper::KmGB; rough (OPS negative); Kmr derivative of Ruokola/71-c | This work |
| YeO9-OC | Δ(wzx-wbcL)::KmGB; OC negative; derivative of Ruokola/71 | This work |
| YeO9-c-OC | Δ(wzx-wbcL)::KmGB; OC negative; derivative of Ruokola/71-c | This work |
| YeO9-OCP | Phage φR1-37-resistant spontaneous low-OC-expressing derivative of Ruokola/71 | This work |
| YeO9-galF | ΔgalF::KmGB; derivative of Ruokola/71; Kmr | This work |
| YeO9-c-galF | ΔgalF::KmGB; derivative of Ruokola/71; Kmr | This work |
| Plasmids | ||
| pRV1 | Suicide vector; Clmr | 68 |
| pRV1-galF | PvuII fragment of O:9 OPS gene cluster containing the galF gene cloned into pRV1 | This work |
| pRV1-galF-Nsi | pRV1-galF with the galF internal NsiI fragment deleted | This work |
| pRV1-galF-Nsi::GB | pRV1-galF with the galF internal NsiI fragment replaced by KmGB | This work |
| pRV1-galU | MscI fragment of O:9 OPS gene cluster containing the galU gene cloned into pRV1 | This work |
| pRV1-galU-del | pRV1-galU with a deletion in the galU gene | This work |
| p87/I | pHC79 carrying a genomic fragment of Ruokola/71-c containing the OPS gene cluster | This work |
| p46/III | pHC79 carrying a genomic fragment of Ruokola/71-c containing the OPS gene cluster | This work |
| p73/II | pHC79 carrying a genomic fragment of Ruokola/71-c containing the OPS gene cluster | This work |
| p77/II | pHC79 carrying a genomic fragment of Ruokola/71-c containing the OPS gene cluster | This work |
| pKK232-8 | Promoter trapping vector; Ampr | GE Healthcare |
| pPSL | 10-kb PstI fragment of Ruokola/71 genomic DNA cloned into pUC18 | This work |
| pWBO:9Sac | SacI fragment of pPSL | This work |
| pWBO:9SacGB | KmGB cloned into the EcoRV site within the per gene in pWBO:9Sac | This work |
| pRV1-WBO:9SacGB | 4-kb PvuII fragment of pWBO:9SacGB cloned into the EcoRV site of suicide vector pRV1 | This work |
| pHC79 | Cosmid cloning vector; Ampr | |
| pUC18 | Plasmid cloning vector; Ampr | |
| pUC4K | Source of the KmGB cassette; Ampr Kmr | Pharmacia |
| pRV19-GB | Suicide vector to inactivate OC gene cluster; Δ(wzx-wbcL)::KmGB; Clmr Kmr | 68 |
| Bacteriophages and bacteriocins | ||
| φR1-37 | OC-specific bacteriophage | 46, 67 |
| Enterocoliticin | OC-specific bacteriocin | 69, 70 |