TABLE 2.
Primers used in this work
| Primer name | Sequence | 5′ position | Direction, accession no., use |
|---|---|---|---|
| Per-2 | GGMGATGAAGTBATTGTWCCRAC | 7148 | Forward, AJ605741, degenerate primer for amplifying per gene fragment of Ruokola/71-c for a probe |
| Per-4 | GTYTTRTTYCCAAARAARCTAAA | 7482 | Reverse, AJ605741, degenerate primer for amplifying per gene fragment of Ruokola/71-c for a probe |
| galU-f | GTTGTCATCGACGGGATATG | 4700 | Forward, AJ605741, plasmid PCR to delete part of galU gene |
| galU-r | ATTGAATCAGGGGCTTATCG | 3919 | Reverse, AJ605741, plasmid PCR to delete part of galU gene, cloning of the galU upstream promoter |
| PSL-F7a | TATCAACTGCAACTGCAAAG | 3454 | Forward, AJ605741, cloning of the galU upstream promoter |
| F7 | CTGAATCATTACTTCGAGTG | 3550 | Forward, AJ605741, cloning of the galU upstream promoter |
| galF-F | ACCGGGCAATCCTATAACTG | 5532 | Forward, AJ605741, cloning of the gmd upstream promoter |
| pSL-7B | GTTGTAACATCATCCATTGC | 6527 | Reverse, AJ605741, cloning of the gmd upstream promoter |
| pSL-7600Ra | TGTTGACAATGGCCATATGA | 8552 | Forward, AJ605741, cloning of the wzm upstream promoter |
| pSL-6A | AATTTATGAAAAGGCATGCC | 7528 | Reverse, AJ605741, cloning of the wzm upstream promoter |