TABLE 3.
Specific CAT activities of C. glutamicum strains carrying different adhA promoter fragments in plasmid pET2
| Strain | Specific CAT activity (U/mg protein)a
|
||||
|---|---|---|---|---|---|
| EtOH | Glc | EtOH/Glcb | Ac | EtOH/Acb | |
| WT C. glutamicum(pET2-adhAp1) | 6.89 ± 0.88 | 1.57 ± 0.02 | 1.68 ± 0.04 | 0.43 ± 0.03 | 0.61 ± 0.03 |
| WT C. glutamicum(pET2-adhAp2) | 1.33 ± 0.08 | 0.31 ± 0.01 | 0.18 ± 0.09 | 0.09 ± 0.03 | 0.15 ± 0.02 |
| WT C. glutamicum(pET2-adhAp3) | <0.01 | <0.01 | <0.01 | ND | ND |
| C. glutamicum RG1(pET2-adhAp1) (ramB-negative) | 7.18 ± 0.04 | 3.37 ± 0.11 | 3.35 ± 0.02 | 1.39 ± 0.03 | 1.65 ± 0.03 |
| C. glutamicum RG1(pET2-adhAp2) (ramB-negative) | 0.83 ± 0.06 | 0.24 ± 0.02 | 0.26 ± 0.01 | 0.07 ± 0.01 | 0.09 ± 0.01 |
| C. glutamicum RG2(pET2-adhAp1) (ramA-negative) | NG | 0.01 | 0.01 | NG | NG |
| C. glutamicum RG2(pET2-adhAp2) (ramA-negative) | NG | <0.01 | <0.01 | NG | NG |
a
The strains were cultured in minimal medium containing 1% ethanol (EtOH), 1% glucose (Glc), Glc plus EtOH (1% each), 1% acetate (Ac), or Ac plus EtOH (1% each). The values are means ± standard deviations of the results of three independent experiments. ND, not determined; NG, not grown.
b
In medium containing ethanol plus glucose and ethanol plus acetate, the cells showed biphasic growth behavior with preferential utilization of glucose or acetate, respectively. Cells were harvested in the first exponential phase of growth.