FIG. 3.

Phenotypes of the pcgC mutant and the complemented strains as analyzed by silver-stained PAGE (A) and Western blotting probed with TEPC-15 (anti-PCho) antibody (B). Equal amounts of proteinase K-treated whole-cell lysates (panel A) or untreated whole-cell lysates (panel B) of the following strains were run as follows: lane 1, wild-type strain VP161; lane 2, parent strain AL435; lane 3, pcgC mutant (AL571); lane 4, pcgC mutant harboring vector pAL99 (AL831); lane 5, complemented mutant (AL829).