TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Details and genotype | Reference or source |
|---|---|---|
| E. coli | ||
| SM10 λpir | Strain for propagation of pUA826 and its derivatives; thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km | 31 |
| AL562 | E. coli SM10 λpir containing pAL297 | This study |
| DH5α | General laboratory cloning strain; deoR endA1 gyrA96 hsdR17(rK− mK+) recA1 relA1 supE44 thi-1 Δ(lacZYA-argF)U169 φ80lacZΔM15 F− | Bethesda Research Laboratories |
| P. multocida | ||
| VP161 | Serotype A:1 wild-type strain isolated from a chicken in Vietnam | 40 |
| AL435 | VP161 carrying a Tn916 insertion in the pml417 gene; still fully virulent | This study |
| AL571 | PCho−; pcgC mutant of AL435 | This study |
| AL829 | PCho+; AL571 pcgC mutant complemented with pAL293 | This study |
| AL831 | PCho−; AL571 pcgC mutant harboring pAL99 | This study |
| Plasmids | ||
| pAL99 | P. multocida expression vector; cloned genes are expressed from the strong constitutive P. multocida tpiA promoter | 13 |
| pUA826 | Mob+, R6K replicon, Apr Strepr Specr | 1 |
| pAL297 | Internal fragment of pcgC cloned into pUA826 | This study |
| pAL293 | Intact copy of pcgC cloned into the P. multocida expression vector pAL99 | This study |
| pPCR2.1 | E. coli cloning vector; Apr Kanr | Clontech |