TABLE 3.
O-deacylated LPS (LPS-OH) from P. multocida VP161 wild-type and mutant strainsa
| Strain | CE-ES-MS data
|
Proposed composition | |||||
|---|---|---|---|---|---|---|---|
| [M-4H]4− | [M-3H]3− | [M-2H]2− | Observed molecular ion | Calculated molecular ion | Relative intensity | ||
| AL435 | 743.8 | 991.9 | 1,488.6 | 2,979.1 | 2,977.6 | 1.0 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH |
| 749.4 | 999.2 | 1,499.4 | 3,001.0 | 2,999.5 | 0.7 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH | |
| 780.2 | 1,040.5 | 3,124.6 | 3,122.6 | 0.3 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH | ||
| AL571 | 620.7 | 828.0 | 1,242.0 | 2,486.5 | 2,485.3 | 1.0 | 2Hex, 4Hep, 2Kdo, lipid A-OH |
| 626.4 | 835.2 | 1,253.0 | 2,508.5 | 2,507.3 | 1.0 | 3Hex, 4Hep, Kdo-P, lipid A-OH | |
| 657.0 | 876.1 | 1,314.5 | 2,631.3 | 2,630.3 | 0.5 | 3Hex, 4Hep, Kdo-P-PEtn, lipid A-OH | |
| 666.0 | 889.2 | 2,669.3 | 2,669.4 | 0.2 | 4Hex, 4Hep, Kdo-P, lipid A-OH | ||
| AL829 | 743.7 | 992.1 | 1,488.1 | 2,978.6 | 2,977.6 | 1.0 | 2PCho, 3Hex, 4Hep, 2Kdo, lipid A-OH |
| 749.2 | 999.3 | 1,499.3 | 3,000.7 | 2,999.5 | 0.8 | 2PCho, 4Hex, 4Hep, Kdo-P, lipid A-OH | |
| 780.0 | 1,040.2 | 1,560.8 | 3,123.6 | 3,122.6 | 0.3 | 2PCho, 4Hex, 4Hep, Kdo-P-PEtn, lipid A-OH | |
| AL831 | 620.7 | 827.8 | 1,242.3 | 2,486.6 | 2,485.3 | 0.7 | 2Hex, 4Hep, 2Kdo, lipid A-OH |
| 626.3 | 835.1 | 1,253.0 | 2,508.3 | 2,507.3 | 0.7 | 3Hex, 4Hep, Kdo-P, lipid A-OH | |
| 656.9 | 876.2 | 1,314.9 | 2,631.7 | 2,630.3 | 1.0 | 3Hex, 4Hep, Kdo-P-PEtn, lipid A-OH | |
a
Negative-ion CE-ES-MS data and proposed composition of O-deacylated LPS (LPS-OH) from the P. multocida VP161 wild-type and mutant strains are shown. Average molecular mass units were used for the calculation of molecular weight based on the proposed composition, as follows: lipid A, 952.00; Hex [hexose], 162.15; Hep [heptose], 192.17; Kdo [3-deoxy-d-mannooctulosonate], 220.18; Kdo-P [3-deoxy-d-mannooctulosonate phosphate], 300.13; PEtn [phosphoethanolamine], 123.05; PCho, 165.05.