Figure 1.

Gro/TLE1 interacts specifically with AML1 and AML2 in the yeast interaction trap assay. Interactions between the AML coding regions fused to an activation domain (AD) and Gro/TLE1 proteins fused to LexA-DNA binding domain. LexA-β delineates LexA fused to Cbfβ. LexA-Dmcdc2 served as a negative control. The numbers indicate β-galactosidase activity resulting from reporter activation. + designates relative strength of blueness on X-gal indicator plates based on analyses of at least 8–10 colonies; ++ indicates >20 β-galactosidase units. − designates white colonies, indicative of absolute failure to activate the lacZ reporter gene. The assay configuration was dictated by the observation that LexA-AML constructs alone activated the reporter.