Figure 3.

TLE1 repress TCRα and TCRβ activity in Jurkat cells. Jurkat cells (2.5 × 10⁶) were transfected by SuperFect for 7–8 hr with wild-type or mutated (marked by × at the top panel) TCRβ, 2 μg (A), or TCRα, 1 μg (B) reporters, with or without 1 μg of TLE1 expression vector. Data presented are the average of five (TCRβ) and six (TCRα) independent transfections carried out in duplicate. Control values of reporter lacking TCR enhancer (5–8%) were substracted. (C) A scheme summarizing the various interactions of AML/CBFα with the adjacently bound factors Ets, Myb, and C/EBP, as well as with the coactivators (arrows) p300/CBP and ALY, and with the negative regulators (bar) Ear 2 and TLE.