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TLE1 abrogates LEF- and β-catenin-dependent activity of TOPFLASH. 293 cells (0.5 × 106) were transfected by CaPO4 for 24 hr with 0.5 μg of reporter plasmids containing three LEF-1 binding sites (TOPFLASH) or three mutated LEF-1 binding sites (marked by × at the top panel, FOPFLASH), together with 2 μg of expression plasmid of LEF-1 and β-catenin, in the presence or absence of 2 μg of TLE1 expression vector.
