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Agarose gel (2%) electrophoresis showing the first and second rounds of the group 1 to group 5 (Gp1 to Gp5) multiplex nested PCR products using a mixture of pathogens as a template. M, marker (100-bp ladder); RSV, hRSV; RV, hRV; EV, hEV; MPV, hMPV; Chl, C. pneumoniae; Leg, L. pneumophila; Myc, M. pneumoniae.
