TABLE 2.
Characterization of O-island genetic content for non-O157 STEC strains isolated from the same patient with hemolytic uremic syndromea
| Strain | Serotype | Presence of stx1 | stx2 variant | Presence of eae | espZ allele | Presence of gene
|
Presence of fimbrial OIb
|
||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| OI-71
|
OI-122
|
OI-36
|
1 | 47 | 141 | 154 | |||||||||||||||
| nleA | nleF | nleH1 | efa | pagC | sen | nleB | nleE | nleB2 | nleC | nleH2 | nleD | ||||||||||
| 06-5231 | O55:H7 | − | stx2d | + | γ1 | − | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| 06-5121 | O177:NM | − | stx2d, stx2c | + | β1 | + | + | − | − | − | + | + | + | − | − | − | + | − | − | − | − |
a
The methods utilized were previously described by Karmali et al. (7) and by B. K. Coombes and M. A. Karmali (unpublished).
b
Fimbrial OI coding sequences screened using PCR and Southern hybridization: for OI-01, Z0024; for OI-47, Z1528, Z1530, Z1531, Z1533, Z1534, Z1535, Z1536, Z1537, Z1538, Z1539, Z1540/41, Z1542, Z1548, and Z1556; for OI-141, Z4965, Z4966, and Z4971; for OI-154, Z5220, Z5221, and Z5225 (lpfA).