TABLE 3.
Biological features and incidence of a typical phenotype of nine species of Malassezia
| Species identified by molecular biological analysis | No. of strains | Colony characteristic on CHROM (% of incidence)a
|
Growth characteristic (% of incidence)b
|
Catalase reaction | |||||
|---|---|---|---|---|---|---|---|---|---|
| Size | Color/morphology | Precipitate | SDA | TE slant | EL slant | 40°C | |||
| M. pachydermatis | 43 | Large (100) | Pale pink/smooth (100) | + (100) | Growth (95.3)d | Growth and produced a black zone (95.3)d | Growth (95.3)d | NT | + (100) |
| M. sympodialis | 84 | Large (100) | Pale pink/smooth (100) | + (100) | No growth (100) | Growth and produced a black zone (100) | No growth (100) | NT | + (100) |
| M. globosa | 14 | Small (100) | Purple/smooth (100) | + (100) | No growth (100) | No growth and no change (100) | No growth (100) | NT | + (100) |
| M. dermatis | 5 | Large (100) | Pale pink to purple/smooth (100) | + (100) | No growth (100) | Growth and no change (100) | No growth (100) | NT | + (100) |
| M. furfur | 41 | Large (100) | Pale pink/wrinkled (100) | − (97.6)c | No growth (100) | Growth and produced a black zone (100) | Growth (85.4) | NT | + (100) |
| M. slooffiae | 108 | Small (100) | Pale pink/smooth (100) | − (100) | No growth (100) | Growth and no change (65.7)e | No growth (98.1) | Growth (84.3)e | + (100) |
| M. obtusa | 4 | Medium (100) | Pink/rough (100) | − (100) | No growth (100) | No growth but produced a black zone (100) | No growth (100) | NT | + (100) |
| M. restricta | 71 | Small (100) | Pink/smooth (100) | − (100) | No growth (100) | No growth and no change (100) | No growth (100) | NT | − (100) |
| M. japonica | 2 | Large (100) | Pink/smooth (100) | − (100) | No growth (100) | Growth and produced a black zone (100) | No growth (100) | No growth (100) | + (100) |
a
Incubated at 32°C for 4 to 7 days.
b
Incubated at 32°C for 4 days. NT, not tested; +, positive; −, negative.
c
Only one strain of fresh clinical isolate produced a precipitate.
d
Only two strains of fresh clinical isolates did not grow on SDA and EL and did not produce a black zone on TE.
e
Thirty-seven strains of fresh clinical isolates produced a black zone on TE, and 17 strains did not grow on modified Leeming and Notman agar at 40°C for 4 days.