TABLE 1.
Triplicate results of Trypanozoon PCR-oligochromatography on a set of 23 DNA samples
| Sample (amt [fg]) | Expected result | No. of positive results (performed in triplicate) at laboratory:
|
|||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | ||
| T. brucei brucei AnTat 2.2 | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| Human DNA | Negative | 0 | 0 | 0 | 1 | 0 | 0 |
| T. brucei brucei (2.5) | Positive | 3 | 3 | 0 | 3 | 3 | 3 |
| T. brucei brucei (5) | Positive | 3 | 3 | 0 | 3 | 2 | 3 |
| T. brucei brucei (10) | Positive | 3 | 3 | 0 | 3 | 0 | 3 |
| T. brucei brucei (20) | Positive | 3 | 3 | 3 | 3 | 2 | 3 |
| T. brucei brucei (80) | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| T. brucei brucei (320) | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| T. brucei brucei (1,280) | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| T. brucei gambiense LiTat 1.3 | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| T. brucei rhodesiense AnTat 25.1 | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| T. evansi RoTat 1.2 | Positive | 3 | 3 | 3 | 3 | 1 | 3 |
| T. equiperdum OVI | Positive | 3 | 3 | 3 | 3 | 3 | 3 |
| T. brucei gambiense AnTat 9.1 | Positive | 3 | 3 | 3 | 1 | 0 | 3 |
| T. congolense TRT 17 | Negative | 0 | 0 | 0 | 0 | 2 | 0 |
| T. vivax ILRAD 700 | Negative | 0 | 0 | 0 | 0 | 0 | 2 |
| Theileria parva | Negative | 0 | 0 | 0 | 0 | 1 | 0 |
| Leishmania sp. | Negative | 0 | 0 | 0 | 0 | 2 | 0 |
| Plasmodium sp. | Negative | 0 | 0 | 0 | 0 | 3 | 2 |
| Schistosoma sp. | Negative | 0 | 0 | 0 | 1 | 0 | 0 |
| Bovine DNA | Negative | 0 | 0 | 0 | 0 | 0 | 1 |
| Trypanosoma cruzi | Negative | 0 | 0 | 0 | 0 | 3 | 0 |
| Trypanosoma rangeli | Negative | 0 | 0 | 0 | 0 | 3 | 0 |