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Standard curve generated by real-time PCR. Serial dilutions of each viral plasmid standard (•), ranging from 8 to 8 × 107 copies per reaction mixture, were used to generate the standard curve. The CT values that corresponded to the PCR cycle number were plotted against the copy number of each viral standard. The slope of the line was −3.57, the y intercept was 40.2, and the correlation coefficient was 99.7%.
