FIG. 3.

Effect of glycosylation on the expression level and on mild heat extraction of AIDA-I. (A) Whole-cell extracts of bacteria bearing an empty vector (−) or expressing unglycosylated AIDA-I (Aida) or glycosylated AIDA-I (Aida/Aah) were separated by SDS-PAGE and probed by immunoblotting with antiserum against the HisG tag fused to mature AIDA-I. (B) Bacteria harboring the same plasmids were heat extracted and the extracts recovered by centrifugation prior to separation by SDS-PAGE and Coomassie blue staining. MW, molecular weight.