Figure 1.

Expression of cytosolic PGK in bloodstream trypanosomes is toxic. Trypanosomes expressing the Tet repressor and containing integrated copies of plasmids pHD 735,736, or 777 were grown in the presence or absence of tetracycline (5 μg/ml). All cultures were started at 10⁵ cells per ml. The nature of the inducible gene and the presence or absence of tetracycline in the medium for each symbol are indicated on the right.