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The toxicity of cytosolic PGK is dose-dependent. Bloodstream trypanosomes containing the Tet repressor and pHD 531 (encoding PGKB) were treated with various doses of tetracycline. (a) Digitonin was used to fractionate cells (107) 24 h after tetracycline addition, and PGK was detected by Western blot using a polyclonal antibody to glycosomal PGK, which also detects the cytosolic enzyme. S, supernatant; P, pellet. (b) Growth curves.
