FIG. 3.

Steady-sate levels of Pla are reduced by mutations inactivating crp and cyaA. The following strains were assayed: KIM8-3002 (pPCP1⁻), KIM5-3001 (wild type) (WT), GY5475 (crp), GY5523 (crp, vector control), GY5524 (crp, vector + crp), GY5476 (cyaA), GY5585 (cyaA, vector control), and GY5586 (cyaA, vector + cyaA). A Western blot analysis of whole-cell lysates probed with polyclonal anti-Pla antibody was performed. The locations of the α and β forms of Pla are indicated on the right. Two cross-reactive proteins that appeared in the pPCP1⁻ control are indicated by asterisks. For each sample, an amount equivalent to 1 ml of culture at an OD₆₀₀ of 0.1 was loaded.