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Identification of the +1 transcription start site by primer extension analysis. Total RNA was isolated from M. smegmatis cells transformed with pSdps1 and grown for 72 h in 0.02% glucose-supplemented MB7H9 medium. The cDNA was synthesized and run in lane P, along with a sequencing reaction of the top strand with a reverse primer represented by C, T, A, and G. The sequence is shown on the right, and the +1 site is circled.
