FIG. 3.

(A) Promoter sequence as identified by site-directed mutagenesis of pSdps200; point base mutations in the −10 and −20 regions are shown in boldface and underlined. (B) Effects of specific mutations (first, third, and sixth bases) in −10 and −20 upstream sequences from the +1 site of the dps gene on promoter activity. Differential lacZ expression in liquid cultures of M. smegmatis transformed with variants of pSdps200 is shown. The error bars indicate standard deviations. The cells were grown in MB7H9 medium supplemented with 0.02% glucose and harvested at 72 h.