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. 2007 Oct 12;189(24):8961–8972. doi: 10.1128/JB.01365-07

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Copyright © 2007, American Society for Microbiology

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FIG. 3. — Inhibition in the membrane insertion of Pf3 P2 and F_oC at the nonpermissive temperature in a YidC(C423R) mutant. (A) Protease accessibility of Pf3 P2 in the CS YidC(C423R) mutant. JS7131 bearing both pACYC184-YidC(C423R) and pMS119-Pf3 P2 was grown and analyzed as described in Materials and Methods. As a control, protease accessibility of Pf3 P2 was tested in the JS7131 strain. Pf3 P2 was detected with immunoprecipitation with anti-Lep. (B) Protease accessibility of FoC in the CS YidC(C423R) strain. JS7131 transformants expressing CS YidC(C423R) and F_oC were analyzed as described in Materials and Methods. (C) Signal peptide processing of PC-Lep to C-Lep in the CS YidC(C423R) strain. In addition, insertion of PC-Lep was analyzed in JS7131 grown under YidC depletion conditions (Glu). (D) Signal peptide processing of preCyoA-N-P2 in the CS YidC(C423R) strain. (E) Signal peptide processing of −3M-PC-Lep in the CS YidC(C423R) strain.