FIG. 4.
Membrane insertion of the tested substrates occurs efficiently at 30°C in the parent strain. MC1060 cells expressing Pf3 P2 (A) or FoC (B) were analyzed by protease accessibility. As a control, we show protease mapping of Pf3 P2 in the JS7131 strain under YidC depletion conditions. MC1060 cells expressing PC-Lep (C), CyoA-N-P2 (D), and −3M-PC-Lep (E) were analyzed for signal peptide processing. JS7131 with pACYC184-yidCwt was used to examine signal peptide processing of PC-Lep (F) and −3M-PC-Lep (G). To show the position of the precursor protein, insertion of PC-Lep, P-CyoA-N-P2, and −3M-PC-Lep was analyzed in strain JS7131 grown under YidC depletion conditions (Glu).