FIG. 7.
Effect of YidC depletion on the accumulation of YidC-dependent substrates at 30°C. (A) The levels of YidC are reduced by growth in the JS7131 strain with glucose. E. coli strain JS7131 was grown in LB medium with 0.2% arabinose, and then the cells were shifted to the glucose condition (LB medium with 0.2% glucose) for the indicated depletion time. (B) Signal peptide processing of wild-type PC-Lep at various YidC depletion times. PC-Lep was detected with immunoprecipitation using anti-Lep antiserum. (C) Protease mapping of Pf3 P2 in the JS7131 strain. (D) Signal peptide processing of −3M-PC-Lep in the YidC depletion strain. (E) Signal peptide processing of CyoA-N-P2 at various YidC depletion times. (F) Protease mapping of FoC at various YidC depletion times. FoC was detected without immunoprecipitation.