FIG. 8.
CS suppressor phenotype and membrane insertion of Pf3 coat and FoC is not affected. (A) CS suppressor phenotype of the YidC mutants. The JS7131 strains bearing the CS suppressor YidC(C423R L426E) and YidC(T362E C423R) encoded on a low-copy-number plasmid were streaked on LB plates containing glucose and incubated at 30°C overnight. Each half-plate contains four streaks from four individual colonies bearing the same construct. The CS YidC(423R) strain was streaked out as a control at 30°C and 37°C. (B) Protease accessibility of Pf3 P2 in the CS suppressor YidC(C423R L426E) mutant. Pf3 P2 was detected with immunoprecipitation with anti-Lep antiserum. (C) Protease accessibility of FoC in the CS suppressor YidC(C423R L426E) mutant. (D) Other CS mutants were obtained by introducing single mutations within YidC. Single arginine mutations were introduced at positions 421, 422, 424, and 425 individually on pACYC184-YidC. The JS7131 strain bearing the single arginine YidC mutant plasmid was streaked on LB plates containing glucose and incubated at 30°C and 37°C overnight (left panel). Lysine, proline, and glutamic acid were introduced at position 423 individually on pACYC184-YidC. The JS7131 strain bearing the single YidC mutant plasmid was streaked on LB plates containing glucose and incubated at 30°C or 37°C overnight (right panel).