FIG. 2.

Schematic representation of gelatinase proteolytic processing. The gelE gene encodes a 509-amino-acid protein with a probable signal peptide (S.P.) that is 29 amino acids long. The propeptide sequence extends from residue 30 to residue 191, and the mature gelatinase starts at residue V192. The predicted masses of the mature protein and C-terminal peptide were determined with the ExPASy pI-M_w tool. The molecular weight of the mature protein purified from culture supernatants was determined by mass spectrometry.