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Autolysis assay with FA2-2 strains expressing gelatinase. The autolysis assay was carried out with FA2-2 strains carrying plasmids pML28 (vector alone) (•), pML29 (GelE wild type) (▴), pML34 (GelE-CT14aa) (▪), and pML41 (GelE Q306P) (♦). Samples were analyzed at 30-min intervals as described in Materials and Methods. OD600, optical density at 550 nm; T0, time zero.
