FIG. 7.

Analysis of GelE E137Q protein secretion and biofilm formation. The culture supernatants were concentrated 20-fold and loaded on a 12% polyacrylamide gel for Coomassie blue staining (A) or for Western blot analysis using anti-C-terminal His tag antibody (B). The molecular weight standards were the New England Biolabs broad range protein marker (A) and the broad range prestained protein marker (B). The arrowhead indicates the full-length GelE protein band. (C) Analysis of biofilm formation by FA2-2 strains carrying plasmids expressing wild-type GelE (pML29), GelE E137Q (pML44), and His-tagged GelE E137Q (pML45). The control strain carried only the vector pML28. The error bars indicate the standard deviations. OD₅₅₀, optical density at 550 nm.