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. 2007 Oct 5;189(24):8835–8843. doi: 10.1128/JB.01311-07

TABLE 1.

E. faecalis strains used in this study

Strain Relevant genotype Relevant phenotype Origin
V583 Parental GelE+ Clinical isolate
FA2-2 fsrC GelE− Lab strain
EF 129 fsrC aphA-3 promoter GelE− Spcr pML28 → FA2-2
EF 130 fsrC aphA-3 promoter-gelE GelE++ Spcr pML29 → FA2-2
EF 131 fsrC aphA-3 promoter-gelE (His tag) GelE++ Spcr pML33 → FA2-2
EF 132 fsrC aphA-3 promoter-gelE (C-terminal 14 amino acids) GelE++ Spcr pML34 → FA2-2
EF 133 fsrC aphA-3 promoter-gelE (C-terminal 14 amino acids, His tag) GelE++ Spcr pML37 → FA2-2
EF 134 fsrC aphA-3 promoter-gelE (C-terminal 13 amino acids, His tag) GelE++ Spcr pML42 → FA2-2
EF 135 fsrC aphA-3 promoter-gelE (C-terminal 13 amino acids, His tag) GelE++ Spcr pML43 → FA2-2
EF 136 fsrC aphA-3 promoter-gelE(E303P) GelE++ Spcr pML38 → FA2-2
EF 137 fsrC aphA-3 promoter-gelE(D304P) GelE++ Spcr pML39 → FA2-2
EF 138 fsrC aphA-3 promoter-gelE(I305P) GelE++ Spcr pML40 → FA2-2
EF 139 fsrC aphA-3 promoter-gelE(Q306P) GelE+ Spcr pML41 → FA2-2
EF 140 fsrC aphA-3 promoter-gelE(E137Q) GelE− Spcr pML44 → FA2-2
EF 141 fsrC aphA-3 promoter-gelE(E137Q) (His tag) GelE− Spcr pML45 → FA2-2