TABLE 2.

Plasmids used in this study

Vector	Description	Predicted gelatinase C-terminal sequence
pML28a	Shuttle vector with aphA-3 promoter
pML29b	GelE	GAKEDIQVNQPSESVLVNE
pML33	GelE plus His6 tag	GAKEDIQVNQPSESVLVNELEHHHHHH
pML34	GelE without C-terminal 14 amino acids	GAKED
pML37	GelE without C-terminal 14 amino acids plus His tag	GAKEDLEHHHHHH
pML42	GelE without C-terminal 13 amino acids plus His tag	GAKEDILEHHHHHH
pML43	GelE without C-terminal 12 amino acids plus His tag	GAKEDIQLEHHHHHH
pML38	GelE with glutamic acid-to-proline substitution at position 303	GAKPDIQVNQPSESVLVNE
pML39	GelE with aspartic acid-to-proline substitution at position 304	GAKEPIQVNQPSESVLVNE
pML40	GelE with isoleucine-to-proline substitution at position 305	GAKEDPQVNQPSESVLVNE
pML41	GelE with glutamine-to-proline substitution at position 306	GAKEDIPVNQPSESVLVNE
pML44	GelE with glutamate at position 137 mutated to Gln	GAKEDIQVNQPSESVLVNE
pML45	GelE with glutamate at position 137 mutated to Gln plus His6 tag	GAKEDIQVNQPSESVLVNELEHHHHHH

^aDerivative of pAT28 (39) carrying the aphA-3 promoter as a 369-bp EcoRI-BamHI fragment (12).

^bSee reference 12.