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. 2007 Oct 5;189(24):8922–8927. doi: 10.1128/JB.00925-07

FIG. 2.

FIG. 2.

Demonstration of ispD essentiality for M. tuberculosis H37Rv survival. Double-crossover strains were isolated in the wild-type and merodiploid backgrounds and analyzed using PCR primers IspDint1 and IspDint2 to amplify ispD. All strains in the wild-type background were wild type. Strains with either a wild-type or deletion allele were isolated in the merodiploid background. Lane M, lamdba HindIII marker; lanes 1 to 5, double-crossover strains generated from the merodiploid background; lane 6, wild-type genomic control; lane 7, delivery vector control. Lanes 1, 2, 4, and 5 show the product expected for a deletion strain, and lane 3 shows the product expected for a wild-type strain.