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. 2007 Oct 5;189(24):8922–8927. doi: 10.1128/JB.00925-07

FIG. 3.

FIG. 3.

Purification of recombinant IspD. Analysis of protein fractions from E. coli transformed with pET28a(+)::Rv3582c is shown. Lane 1, cell lysates prior to IPTG treatment; lane 2, cell lysates after IPTG treatment; lane 3, purified His6-IspD fraction after immobilized metal-affinity chromatography; lane 4, Western blot hybridization analysis of purified IspD using an anti-His antibody; lane M, molecular size markers (kDa). In lane 3, Rv3582c expression was visualized with Coomassie brilliant blue 250R.